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. 2007 Apr 2;104(15):6247–6252. doi: 10.1073/pnas.0701509104

Fig. 1.

Fig. 1.

Purified Wnt3a induces expression of Pitx2, cyclin D2, and other cell cycle regulators in β cells and promotes cell expansion. (A) Expression of Ki67 in Nkx6.1+ cells of purified WT P8 islets stimulated with Wnt3a, vehicle, or Wnt3a + Fz8-CRD. (B) Growth of MIN6 cells stimulated with vehicle only, Wnt3a, or Wnt3a + Fz8-CRD and counted at 24, 48, and 72 h. Each condition was performed in triplicate. (C) MIN6 cells stimulated with Wnt3a or vehicle for 8 h were assayed for BrdU incorporation by immunofluorescence analysis. (A–C) Data are presented as the average ± SEM. All RT-PCR results were normalized to β-actin and are the average value from triplicate experiments. P values are indicated in each graph. (D) Real-time RT-PCR analysis of Pitx2, cyclin D2, cyclin D1, and cdk4 cDNA from purified P8 islets treated with vehicle only, purified Wnt3a, or Wnt3a + Fz8-CRD for 24 h. (E) Real-time RT-PCR analysis of Pitx2, cyclin D2, cyclin D1, and cdk4 cDNA from MIN6 cells treated with vehicle only, purified Wnt3a, or Wnt3a + Fz8-CRD for 24 h.