Table 2.
Osteocyte viability was quantified from 7 μm cryo-sections taken at ≥1.0 mm distance, the region of interest, through each bone core at Time zero (T0) or maintained within the 3-D bioreactor system with or without mechanical stimulation for 7 days (n=5 Patients), 3 days (n=2 Patients) and 27 days (n=1 Patient). The results are expressed as the average number of LDH positive osteocytes per mm2 from n=4 bone cores per treatment group±SEM.
| Patient | Treatment | |||||||
|---|---|---|---|---|---|---|---|---|
| Sex | Age | Length of Experiment (days) | TO | Unloading | TO vs. Unloading p value | Mechanical Stimulation | Unloading vs. Mechanical Stimulation p value | |
| 1 | Female | 34 | 7 | 124.8±8.9 | 25.1±6.0 | p=4.4 x 10-6 | 48.9±8.1 | p=0.021 |
| 2 | Female | 65 | 7 | 119±2.9 | 38.7±2.3 | p=8.4 x 10-5 | 74.5±5.2 | p=3.6 x 10-5 |
| 3 | Male | 46 | 7 | 160±11 | 41±12.4 | p=0.0004 | 84.4±6.5 | p=0.0076 |
| 4 | Female | 90 | 7 | 113.5±9.2 | 20.3±3.9 | p=3.3 x 10-6 | 64.4±11 | p=0.00032 |
| 5 | Male | 55 | 7 | 138.3±14.1 | 29.1±5.1 | p=2.2 x 10-5 | 46.2±13.9 | p=0.03 |
| 6 | Male | 75 | 3 | 95.5±7.5 | 33.7±2.9 | p=0.00025 | 70.9±3.1 | p=0.018 |
| 7 | Female | 47 | 3 | 162±1.6 | 65.8±4.6 | p=4.5 x 10-6 | 106±4.91 | p=0.019 |
| 8 | Male | 65 | 27 | 118.5±27 | 28.3±1.4 | p=0.0007 | 48±9 | p=0.009 |