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. 2006 Oct 1;15(5):583–595. doi: 10.1111/j.1365-2583.2006.00679.x

Figure 4.

Figure 4

Localization and relative quantification of AmUSP mRNA in mushroom bodies of the bee brain. A. The mushroom bodies are seen in transverse section through the brain of a 1 day old hybridized with probe for AmUSP. B. Mushroom bodies of a > 21 day old forager brain hybridized with the same probe as A. Asterisk indicates the region of the inner compact Kenyon cells. K cbs, Kenyon cell bodies; Oc, ocelli; Pt, protocerebrum. Scale bars = 100 µm. C. qRT-PCR analysis of AmUSP expression in individual mushroom bodies of 7 day pupae, 1 day workers, new precocious foragers (NPF), experienced precocious foragers (EPF), and foragers older than 21 days (2 genotypes; N = 8 brains per group). Data are means ± SE (converted as relative units to the lowest group mean). P and F-values for a one way anova are indicated. Bars with the same letter indicate means are not significantly different (t-test for LSD P > 0.05).