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. 2007 Feb 28;2007:25935. doi: 10.1155/2007/25935

Figure 2.

Figure 2

Figure 2

Figure 2

Figure 2

Biological activity of GST fusion proteins. (a) Control of homogeneity by 3%–8% (w/vol) SDS-PAGE of 200 ng of each purified protein is shown; (1) molecular ladder, (2) LukF-PV, (3) GST∼LukF-PV, (4) LukS-PV, (5) GST∼LukS-PV. (b) Flow cytometry evaluation of the Ca2+ entry into human PMNs mediated by combinations of wild-type LukS-PV and LukF-PV and GST fusion proteins. (c) Flow cytometry evaluation of the ethidium entry into human PMNs mediated by combinations of LukS-PV and LukF-PV and GST fusion proteins. (d) Hydrodynamic radius of pores formed by WT and fusion proteins in human PMNs determined after a 30-minute incubation of toxins (20 nM of S and 100 nM of F components). Osmotic protection by polyethylene glycol molecules was assessed by variations of the mean FSC (forward light scatter) value in the presence of PEG molecules of different hydrodynamic radii.