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. 2007 Feb 28;2007:25935. doi: 10.1155/2007/25935

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Copyright © 2007 Olivier Joubert et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Oligomers formed by PVL and modified toxins. Oligomers were checked in solution or after recovery from treated human PMNs, 3–8% (w/v) SDS-PAGE and immunoblotting with anti-LukS-PV and anti-LukF-PV affinity-purified rabbit antibodies. Lane 1: LukS-PV + LukF-PV without membranes, lane 2: PMNs only, lane 3: GST∼LukS-PV + GST∼LukF-PV, lanes 4, 5, 6: toxins applied on PMNs membranes and then saponin/glutaraldehyde treated and heated 5 minutes at 100°C as described in materials and methods, lane 4: LukS-PV + GST∼LukF-PV, lane 5: GST∼LukS-PV + LukF-PV, lane 6: GST∼LukS-PV + GST∼LukF-PV.