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Flow cytometry evaluation of ethidium entry into human PMNs for different combinations of purified WT S and F proteins and their oxidized or reduced double mutants. (a) Oxidized LukS-PV mutants were first tested with WT LukF-PV. (b) Oxidized LukF-PV mutants were tested with WT LukS-PV. (c) Oxidized or reduced LukS-PV double mutants were tested with LukF-PV S8C-K20C. (d) Oxidized or reduced LukS-PV double mutants were tested with LukF-PV T5C-T21C.
