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. 2007 Feb 28;2007:25935. doi: 10.1155/2007/25935

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Copyright © 2007 Olivier Joubert et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Determination of the hydrodynamic radius of pores formed by the different combinations of oxidized LukS-PV (20 nM) and LukF-PV (100 nM) mutants 30 minutes after application of toxins to human PMNs. Osmotic protection was assessed by variations of the mean FSC (forward light scatter) value in the presence of polyethylene glycol molecules of different hydrodynamic radii. (a) Oxidized LukS-PV double mutants were tested with LukF-PV S8C-K20Cox. (b) Oxidized LukS-PV double mutants were tested with LukF-PV T5C-T21Cox.