Table 1.
Times (min) to reach (a) 5% and (b) 100% of the Fluo-3 fluorescence characteristic of the cellular entry of Ca2+ due to calcium channels activation by combinations of PVL (taken as reference) and its mutants, n.r.: not reached, n.d.: not determined.
| LukF 40 nM | LukF 2 nM | LukF-T5CT21C | LukF-S8CK20C | |
| LukS 1 nM | 1.25(a) | 3.5(a) | 1.25(a) | 4.5(a) |
| 2.5(b) | 10(b) (95%) | 5(b) | n.r. | |
| LukS-D1CR16C | 6(a) | n.d. | 3.4(a) | n.r. |
| n.r.(b) | 7.5(b) (90%) | n.r. | ||
| LukS-(-1C)R16C | 1.25(a) | n.d. | 1.75(a) | n.r. |
| 2.5(b) | 5(b) | n.r. | ||
| LukS-N2CR16C | 1.25(a) | n.d. | 1.5(a) | 3(a) |
| 2.5(b) | 5(b) | 7.5(b) (95%) | ||