Table 1. Effects of CuCl2 supplementation on MCP 1-induced notochord deformation and albinisma , b .
[MCP]/nM | [CuCl2]/nM | N | Notochord |
Melanisation |
||
% Wavy (%) | Normal (%) | Albino (%) | Normal (%) | |||
0 | 0 | 21 | 0 | 100 | 0 | 100 |
100 | 20 | 0 | 100 | 0 | 100 | |
1000 | 19 | 0 | 100 | 0 | 100 | |
100 | 0 | 20 | 100 | 0 | 0 | 100 |
100 | 20 | 100 | 0 | 0 | 100 | |
1000 | 18 | 100 | 0 | 0 | 100 | |
500 | 0 | 20 | 100 | 0 | 0 | 100 |
100 | 4 | 100 | 0 | 0 | 100 | |
1000 | 0 | Xc | Xc | Xc | Xc | |
1000 | 0 | 19 | 100 | 0 | 0 | 100 |
100 | 8 | 100 | 0 | 75d | 25 | |
1000 | 0 | Xc | Xc | Xc | Xc | |
50000 | 0 | 20 | 100 | 0 | 100 | 0 |
100 | 16 | 100 | 0 | 100 | 0 | |
1000 | 0 | Xc | Xc | Xc | Xc |
aSupplementation with ZnCl2 gave equivalent results, data not shown.
bCuCl2 alone does not induce notochord deformations.
cLethal at high concentrations.
dA mild decrease in pigmentation was observed. N = number of embryos scored.