Modification of the Ste5 N-terminus is cell cycle dependent. Cells (
cdc15-2 or
cdc28-13) harboring Ste5-HA
3 (ΔNLS or ΔNLS+8A) were arrested at 37°C for 3 hr, then transferred to 25°C to resume cycling. Samples were collected at 20 min intervals (0-180 min). As
cdc15 cells arrest with large buds, emergence of small buds was used to follow cell cycle progression (c.f.,
Figure S1D).