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. Author manuscript; available in PMC: 2008 Jan 5.
Published in final edited form as: J Mol Biol. 2006 Sep 20;365(1):18–30. doi: 10.1016/j.jmb.2006.09.044

Figure 4B. Preferential association of unacetylated histones in regions of the SV40 genome lacking RNA Polymerase II in chromosomes undergoing early and late transcription.

Figure 4B

Unfixed SV40 chromosomes were isolated from cells infected with 776 wild type virus for 30 minutes or 48 hours and subjected to an ISFIP/ReChIP analysis with antibodies to hyperacetylated histone H4 and H3 and unacetylated histone H4 and H3 as described in the materials and methods. The samples were amplified with primer sets to the early and late regions. The position of the amplification product from the wild-type 776 DNA is indicated. Lane 1: ISFIP input fraction; lane 2: ChIP with 10 μl of unacetylated histone H4 antibody (ISFIP); lane 3: ChIP with 10 μl of unacetylated histone H3 antibody (ISFIP); lane 4: ReChIP input fraction; lane 5: ChIP with 10 μl of unacetylated histone H4 antibody (ReChIP); lane 6: ChIP with 10 all of unacetylated histone H3 antibody (ReChIP). The PCR products in the ISFIP and ReChIP lanes were amplified from one half of the total amount of DNA obtained from each of the samples. The PCR products in the input lanes were amplified from one fourth of the total amount of DNA present in each of the input samples. Similar results were obtained from at least three separate preparations of SV40 chromosomes for each time point.