Figure 1.

Ikaros expression in long-term self-renewing hematopoietic stem cells. (A) LT-HSC purified from adult bone marrow with the cell surface phenotype Sca-1⁺Lin⁻Thy-1.1^loc-kit⁺ were sorted twice consecutively and then fixed on glass slides. Cells were stained with an affinity-purified, rabbit polyclonal antibody to the C-terminal portion of Ikaros. The secondary staining was done by using an FITC-conjugated, goat anti-rabbit antibody. Red staining (a) represents the Texas Red-conjugated, anti-Sca-1 antibody used in the sort. The punctate staining pattern (b) was seen in all stem cell and lymphoid cell populations analyzed with either the C-terminal antibody or with an affinity-purified antibody to the N-terminal portion of Ikaros. (B) LT-HSC were stained with rabbit preimmune serum followed by secondary staining with goat anti-rabbit^FITC antibody.