Figure 2.

Ikaros RNA splicing isoforms in stem cells and progenitor B and T cell populations. HSC and pro-B cells from adult bone marrow and pro-T cells from adult thymus were double-sorted based on cell surface marker expression (See Materials and Methods). Five or ten cells were cloned by FACS into tubes for RT-PCR. The RT reaction was done by using an Ikaros-specific primer complementary to a portion of exon 7. Two sequential rounds of nested PCR were performed with an aliquot of cDNA using primers localized to exons 1/2 and exon 7. The Southern blot was probed with an internal oligonucleotide complementary to sequence found in exon 1/2. The vertical rectangles on the isoform diagram denote the zinc finger domains within Ikaros.