Table 1.
Summary of Ikaros RNA expression in hematopoietic subsets
| IK-1 | IK-2 | IK-2A/ IK-4* | IK-4A | IK-6 | |
|---|---|---|---|---|---|
| LT-HSC | X | X | |||
| ST-HSC | X | X | X | X | |
| MPP | X | X | 2A | X | |
| Lymphoid progenitor | X | X | X | X | X |
| pro-B | X | X | X(4) | X | X |
| pro-T | X | X | X | X | X |
| Neutrophil† | X | X | 4 | X |
*
For the IK-2A/IK-4 comigrating bands. “X” represents the presence of a band, and 2A or 4 indicates a positive identification by sequence. The IK-4 identified as pro-B was sequenced in an Abelson-transformed pre-B cell line and has been identified in a T cell line by Molnar and Georgopoulos (16). All other X designations come from RT-PCR analyses of normal cells sorted by FACS according to their distinctive phenotype.
†
Neutrophils generally contained only one of the indicated isoforms in five-cell PCRs.