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. Author manuscript; available in PMC: 2008 Jan 23.
Published in final edited form as: FEBS Lett. 2006 Dec 14;581(2):223–232. doi: 10.1016/j.febslet.2006.12.015

Figure 1.

Figure 1

PSF can be recognized by SR phosphorylation-specific mAb 3C5 in mammalian cells. HA-tagged PSF (pCR3.1·PSF-HA), untagged PSF (pCR3.1·PSF) or the empty expression vector (mock) was transiently transfected into Cos-7 cells. After 48 hrs, supernatants from whole cell lysates were collected and immunoprecipitated with an anti-HA monoclonal antibody. Samples were resolved on 10% SDS-PAGE, transferred to a PVDF membrane, and then western blotting was performed with anti-PSF (A) or mAb 3C5 (B). Bands at ∼50 and 25 kD in all samples result from reaction of the anti-mouse secondary antibody against heavy and light immunoglobulin chains.