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. Author manuscript; available in PMC: 2008 Jan 23.
Published in final edited form as: FEBS Lett. 2006 Dec 14;581(2):223–232. doi: 10.1016/j.febslet.2006.12.015

Figure 3.

Figure 3

In vitro phosphorylation of PSF by SRPK1 or Dsk1. 1μM of the indicated bacterially expressed and purified recombinant proteins were incubated with0.5 μM Dsk1 (A) or with the same concentration of SRPK1 (B) in the presence of [γ-32P]ATP at 23°C for 30 min. Samples were resolved by 10% SDS-PAGE and visualized by phosphoimaging. Positions of phosphorylated PSF and autophosphorylated kinase are indicated.