A, Western blot demonstrating over-expression of 12-lipoxygenase (12-Lox) in HT4 cells. pcDNA3.1, empty plasmid control; pcDNA3.1+12-Lox, 12-Lox expression vector. B, 12-Lox over-expression accelerated HCA-induced cell death. Both TCT (0.25μM) as well as BL15 (2.5μM) protected against the toxic effects of 12-Lox. *, lower than group treated with HCA alone; †, HCA induced more toxicity in 12-Lox over-expressing cells than in control cells. TCT, α-tocotrienol.