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. Author manuscript; available in PMC: 2007 Apr 6.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Feb 9;355(2):338–341. doi: 10.1016/j.bbrc.2007.01.194

Figure 1.

Figure 1

Developmental expression of PLM, NCX and α1 subunit of Na+/K+-ATPase in rabbit heart. Membrane fractions were isolated (see method section for details) from Newborn (1–4 day old), 9–11 day old and adult rabbit hearts and run on an 8% gel for NCX and Na+/K+-ATPase, and on a 15% gel for PLM. (A) Sypro Ruby protein blot stain was used to detect the amount of proteins in each lane. 25 μg of protein was loaded in each lane. (B) Protein expression of PLM, NCX and α1 subunit of Na+/K+-ATPase in newborn rabbit is at least 3 fold higher than in adults. (C) Bar graph showing the protein expression of α1 subunit of Na+/K+-ATPase (n=3), NCX (n=5) and PLM (n=5) during different developmental stages of rabbit heart by using image processing and analysis program Image J. The values were normalized to the respective adult values as 1 in each group.