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. Author manuscript; available in PMC: 2007 Apr 6.
Published in final edited form as: Biochem Biophys Res Commun. 2007 Feb 9;355(2):338–341. doi: 10.1016/j.bbrc.2007.01.194

Figure 2.

Figure 2

Immunofluorescence localization of PLM, NCX and α1 subunit of Na+/K+-ATPase in newborn and adult rabbit heart. PLM was visualized by using Cy3 conjugated goat anti-rabbit IgG, NCX by using FITC conjugated goat anti-mouse IgM and α1 subunit of Na+/K+-ATPase by using Cy2 conjugated donkey anti-mouse IgG. All the secondary antibodies were used in 1:300 dilution. (A) Newborn and (B) Adult rabbit myocytes stained with NCX (green) and PLM (red) reveal strong expression of these proteins in the sarcolemma in newborns whereas in the sarcolemma and z-lines in the adult. Co-staining demonstrates a co-localization pattern in neonatal as well as adult rabbit heart. (C) Staining with α-1 antibody shows a sarcolemmal staining pattern in newborns whereas in adult rabbit ventricular myocytes, we find sarcolemmal and z-line staining.