Table 1.

Representative CRPV mutants list

Construct name	Mutation site	Mutation information
E1m#1	E1	CRPVE1 sequence 3128–3170bp replaced by corresponding ROPVE1
E1m#2		CRPVE1 sequence 1999–2017 replaced by corresponding ROPVE1
E2m#1	E2	CRPVE2 sequence 4021–4283bp replaced by corresponding ROPVE2
E2m#2		CRPVE2 sequence 3128–3382bp replaced by corresponding ROPVE2
E5m#1	E5	CRPVE5/E8 double ATG mutant with wild type E8 at NsiI
E5m#2		CRPVE5 ATG mutant with 314bp fragment of HSV-tk gene in the sense orientation at NsiI
E5m#3		CRPV with a deletion of 4300–4344bp in E5
E6m#1	E6	CRPVE6 with new enzyme sites engineered at 326bp/MscI and 476bp/HpaI
E6m#2		CRPV E6 and E7 replaced by ROPVE6 and E7
E6m#3		CRPVE6 fragment 326–476bp replaced by corresponding ROPV fragment
E6m#4		CRPVE6 fragment 326–973bp replaced by corresponding ROPV fragment
E6m#5		CRPVE6 fragment 476–973bp replaced by corresponding ROPV fragment
E7m#1	E7	CRPVE7 point mutation resulting in S21 to G
E7m#2		CRPVE7 point mutation resulting in S21 to D
E7m#3		CRPVE7 point mutation resulting in R57 to S
E7m#4		CRPVE7 replaced by CRPV regressive strain E7
E7m#5		CRPVE7 containing HPV16E7/82–90 epitope LLMGTLGIV
URRm#1	URR	CRPV with 7732–7786 deletion and a mutation in the 8th E2 binding site
URRm#2		Replacement of CRPVURR with ROPVURR at engineered enzyme sites BsiW1/7380 and SacII/152 deletion of URR7671–7798 fragment)
URRm#3
URRm#4		CRPVURR with four additional E2 binding sites at Bg lII
URRm#5		CRPVURR with two copies of ROPVURR 7458–7556bp fragment in sense orientation at BglII
URRm#6		CRPVURR with full length of ROPVURR inserted at Bg lII
URRm#7		CRPVURR with a deletion of most 5′ E2 binding site 7420–7466bp
URRm#8		CRPVURR with insertion of a 314bp fragment of the HSV-tk gene at BglII
URRm#9		CRPVURR with two additional E2 binding sites at BglII
URRm#10		CRPVURR with six additional E2 binding sites at BglII