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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Virus Res. 2006 Oct 30;124(1-2):29–37. doi: 10.1016/j.virusres.2006.09.009

Figure 3. Characterization of HIV-1 U5-PBS complementary to tRNAPhe and tRNATyr.

Figure 3

Panel A. HIV-1 was constructed with mutations in the U5-PBS to be complementary to tRNAPhe or tRNATyr. The viruses were isogenic with NL4-WT except for the designated mutations.

Panel B. Infectivity. The viruses with U5-PBS complementary to tRNAPhe or tRNATyr. The infectivity of these viruses following transfection of proviral genomes into 293T cells was determined. The amount of infectious virus released (IUs) was determined using the JC53-BL assay while the levels of total virus produced were measured using the p24 antigen capture ELISA. The infectivity (IU/p24) for each virus was then compared to the wild type virus. Data presented is from three independent transfections.