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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Virus Res. 2006 Oct 30;124(1-2):29–37. doi: 10.1016/j.virusres.2006.09.009

Figure 5. Sequence comparison of PBS and 3’ terminal nucleotides of tRNALys,3.

Figure 5

Panel A. Interaction of 3’ terminal 18 nucleotides of tRNALys,3 with viruses that have PBS complementary to tRNAThr (NL4-Thr) and tRNAMet (NL4-Met). Stable refers to PBS region in the absence of A-loop modification.

Panel B. Interaction of 3’ terminal nucleotides of tRNALys,3 with virus that have PBS complementary to tRNASer (NL4-Ser), tRNAIle (NL4-Ile) or tRNAPhe (NL4-Phe). Unstable refers to the PBS region that reverts to be complementary to tRNALys,3 following culture. Note that NL4-Ser and NL4-Ile revert even with A-loop modifications, while NL4-Phe-AC replicates poorly with A-loop modification.

Panel C. Interaction of 3’ terminal nucleotides of tRNALys,3 with PBS complementary to tRNATyr (NL4-Tyr). Poor replication means virus does not grow in SupT1 or PBMC under standard conditions. Note that this virus is not resulted by A-loop modification (NL4-Tyr-AC).

Base pair mismatches between PBS and tRNAs for Panels A, B and C are noted by vertical lines.