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. Author manuscript; available in PMC: 2008 Mar 1.

Published in final edited form as: Virology. 2006 Oct 9;359(1):233–242. doi: 10.1016/j.virol.2006.09.009

Virus antigen positive cells are apparent in sciatic nerves at 3 days post-infection of the sciatic nerve with TMEV; staining of a TMEV-injected sciatic nerve is shown in (A). Dark reaction product indicates positive staining (examples shown by white arrowhead). No immunoreactivity was observed in the sciatic nerve of an HBSS-injected mouse (B). Following injection of UV-inactivated virus into the sciatic nerve, viral proteins were not detected by immunohistochemistry (C). Slides were stained using the ABC Methodology as described previously (Drescher, Nguyen et al., 1998). Development was carried out using DAB. Double immunofluorescent staining was performed using markers specific for myelinating cells (D, F, G) combined with identification of TMEV (E) as described in the Methods. Single channel images are shown (D, E) as well as merged images (F, G). Staining is shown for sciatic nerves from FVB mice at 5 days post-inoculation (D–F). A merge image of the uninfected contralateral sciatic nerve is negative for TMEV (G). Controls included staining a normal mouse brain with the antibody to myelinating cells to demonstrate specificity for white matter areas of the brain (H). A DAPI image of the brain is shown for comparison purposes (I). Original magnification X480 (A–C), X1000 (D–G), X80 (H, I).

Virus antigen positive cells are apparent in sciatic nerves at 3 days post-infection of the sciatic nerve with TMEV; staining of a TMEV-injected sciatic nerve is shown in (A). Dark reaction product indicates positive staining (examples shown by white arrowhead). No immunoreactivity was observed in the sciatic nerve of an HBSS-injected mouse (B). Following injection of UV-inactivated virus into the sciatic nerve, viral proteins were not detected by immunohistochemistry (C). Slides were stained using the ABC Methodology as described previously (Drescher, Nguyen et al., 1998). Development was carried out using DAB. Double immunofluorescent staining was performed using markers specific for myelinating cells (D, F, G) combined with identification of TMEV (E) as described in the Methods. Single channel images are shown (D, E) as well as merged images (F, G). Staining is shown for sciatic nerves from FVB mice at 5 days post-inoculation (D–F). A merge image of the uninfected contralateral sciatic nerve is negative for TMEV (G). Controls included staining a normal mouse brain with the antibody to myelinating cells to demonstrate specificity for white matter areas of the brain (H). A DAPI image of the brain is shown for comparison purposes (I). Original magnification X480 (A–C), X1000 (D–G), X80 (H, I).