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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Virology. 2006 Oct 9;359(1):233–242. doi: 10.1016/j.virol.2006.09.009

Figure 3. Real-time RT- PCR demonstrates that PLP mRNA levels peak at day 3 post-injection of the sciatic nerve with TMEV (solid circles).

Figure 3

A 4.9 ± 0.5 -fold relative increase in PLP mRNA levels is evident as early as 2 hours after sciatic nerve injection with TMEV (solid circles; p = 0.02). By day 3 p.i. PLP transcript levels peak, with a 15.7 ± 2.7-fold increase in mRNA levels as compared to uninjected control sciatic nerves (p < 0.032). This increase in PLP mRNA levels begins to subside by day 5 p.i. with virus, but still remain 3.1 ± 0.3-fold higher than baseline levels (p = 0.02). Additional control mice were injected with HBSS (open circles) into the sciatic nerve. No significant alterations in PLP mRNA levels were observed over 5 days. Standardization was performed using GAPDH as a housekeeping gene. Real-time PCR was performed using TaqMan methodology (Methods) on an ABI 7000 SDS Sequence Detection System (Applied Biosystems).