Table 1.

Confirmation of microarray mRNA expression patterns by quantitative real-time RT-PCR (qRT-PCR) or semi-quantitative RT-PCR. mRNA expression levels (mean ± standard error, n=3) relative to young control animals (YC) determined with microarray and by qRT-PCR or semi-quantitative RT-PCR (for Il1α and TNFα). Microarray values are given as ratios relative to normalization and RMA levels derived from Genespring. qRT-PCR values are ratios relative to β-actin (housekeeper gene) expression. For all genes measured, RT-PCR corroborated the rank order of magnitude of expression measured on the microarrays.

		YC		YC LPS		OC LPS		OM LPS
Gene Name	Gene ID	μ Array	qRT-PCR	μ Array	qRT-PCR	μ Array	qRT-PCR	μ Array	qRT-PCR
IL-1α	16175	1.00±0.03	1.00±0.01	1.41±0.04	8.91±1.54	1.28±0.04	5.00±0.24	2.00±0.18	14.7±1.5
IL-1β	16176	1.00±0.05	1.00±0.17	2.01±0.08	4.58±0.48	2.54±0.28	5.47±1.36	4.19±0.71	13.7±5.3
IL-6	16193	1.00±0.07	1.00±0.40	8.57±2.22	10.6±5.18	2.46±0.63	2.62±0.94	13.2±3.5	12.0±3.4
IL1RN	16181	1.00±0.04	1.00±0.43	1.44±0.10	3.91±0.32	2.83±0.47	7.62±1.68	2.69±0.64	7.24±2.89
NFE2L2	18024	1.00±0.04	1.00±0.24	1.08±0.04	1.34±0.39	1.57±0.07	1.92±0.16	1.33±0.08	1.56±0.11
S100A8	20201	1.00±0.06	1.00±0.30	1.92±0.08	13.5±1.0	10.2±2.0	142.±32.	4.02±1.32	49.8±23.8
S100A9	20202	1.00±0.06	1.00±0.36	1.98±0.06	13.2±2.6	7.24±1.29	100.±26.	3.48±0.88	49.9±25.8
STAT1	20846	1.00±0.04	1.00±0.05	1.44±0.04	1.92±0.15	1.80±0.06	2.09±0.08	1.64±0.12	1.95±0.30
TNFα	21926		1.00±0.01		34.3±2.1		24.3±2.3		65.9±11.5

YC = young control group, OC = old control group, OM = old melatonin-supplemented group; YC LPS, OC LPS, OM LPS = YC, OC, or OM groups injected with lipopolysaccharide.