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Effect of cortactin on the CaD-mediated inhibition of acto-S1 ATPase activity*
| Relative ATPase activity | ||
|---|---|---|
| Inhibitor | S1+actin | S1 + actin + cortactin |
| None | 100 | 89 ± 3 |
| H32K | 44 ± 7 | 69 ± 9 |
| CaD | 55 ± 6 | 65 ± 7 |
The Mg2+-activated ATPase activities were measured according to Huang et al. (2003) using 3 μM actin, 0.3 μM S1, 0.5 μM H32K or CaD and 0.5 μM cortactin-B.
