Table 3.

Kinetic and thermodynamic parameters of the interaction of BM3.3 with the specified pMHC ligands

Parameter	pBM1-H-2Kb	pBM8-H-2Kbm8	pBM8Y4D-H2Kbm8
kon (M−1 s−1)	2.8 × 105	9.2 × 104	3.3 × 105
koff (s−1)	1.1	10.3	6.7
KD (μM)	3.6	112	20
ΔG (kcal mol−1)	−7.5±0.1	−5.5±0.03	−6.5±0.1
ΔH (kcal mol−1)	−13.4±0.4	−3.1±0.4	−5.4±0.1
−TΔS (kcal mol−1)	+5.9±0.3	−2.4±0.3	−1.2±0.3
ΔCp (cal mol−1 K−1)	−535±76	−141±7	−108±3.3
Values are reported at 25°C. kon is calculated from the equation kon=koff/KD for the pBM8-H-2Kbm8 and pBM8Y4D-H-2Kbm8 interactions. Except for the pBM8Y4D-H-2Kbm8 ligand, values are reported as the mean of 3 independent experiments±the s.e.m. For the pBM8Y4D-H-2Kbm8 ligand, values are reported as the mean of two independent experiments±the s.e.m.