Figure 2.

Gephyrin interacts with Pin1 in a phosphorylation-dependent manner in vitro. (A) Lysates of HEK 293 cells transfected with gephyrin FLAG were subjected to GST (lane 2) or GST-Pin1 (lane 3) pull-down followed by Western blot with anti-FLAG antibody. (B) Endogenous gephyrin from lysates of a neuroblastoma cell line (SH-SY5Y) was subjected to GST pull-down as described in (A). Western blot analysis was performed with anti-gephyrin antibody. (C) Endogenous gephyrin from SH-SY5Y cells was subjected to pull-down using GST (lane 2), GST-Pin1 (lane 3), GST-Pin1 Y23A (lane 4) and GST-Pin1 C113A (lane 5). Western blot analysis was performed with anti-gephyrin antibody.