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Lack of Pin1 impairs the ability of gephyrin to interact efficiently with GlyRs. Gephyrin-FLAG and GFP-β loop were transfected in MEFs Pin1+/+ (1), MEFs Pin1−/− (2) and MEFs Pin1−/− plus Pin1 (3). Lysates were immunoprecipitated with anti-FLAG (lanes 5, 7 and 9) or anti-MYC as negative control (lanes 4, 6 and 8). Immunoprecipitates were analysed by Western blotting using polyclonal antibodies against gephyrin, GFP and Pin1.
