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. 1990 Aug;56(8):2562–2564. doi: 10.1128/aem.56.8.2562-2564.1990

Isolation of Candida tropicalis auxotrophic mutants.

M A Gleeson 1, L O Haas 1, J M Cregg 1
PMCID: PMC184766  PMID: 2206102

Abstract

An enrichment scheme using nystatin was designed for the isolation of auxotrophic mutants from the diploid-alkane-utilizing yeast Candida tropicalis. A collection of 194 auxotrophs representing 7 phenotypes was isolated. One class of mutants was identified as having a defect in histidinol dehydrogenase activity and a second class of mutants was identified as having a defect in orotidine monophosphate decarboxylase activity. These strains are good candidates to be carrying mutations corresponding to the HIS4 and URA3 genes of Saccharomyces cerevisiae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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