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. 2007 Mar 15;26(7):1902–1912. doi: 10.1038/sj.emboj.7601635

Figure 1.

Figure 1

Transgenic embryonic expression of the Foxk1 promoter fused to lacZ. (A) Schematic outlining the transgenic construct that consists of a 4.6 kb upstream fragment of the Foxk1 gene fused to the lacZ reporter. (B) Whole-mount β-galactosidase expression in limbs, diaphragm and branchial arch derivatives in E11.5 (B), E13.5 (C, E) and E14.5 (D) 4.6 kb Foxk1–lacZ transgenic embryos. (F) Transverse section of the E13.5 embryo reveals β-galactosidase expression in muscle precursor cells of the limb. (G, H) Coexpression of β-galactosidase (α-bgal) and Foxk1 in intercostal muscles of 4.6 kb Foxk1–lacZ transgenic embryos. Low magnification (I) and high magnification (J) of an E13.5 parasaggital section of the Foxk1 promoter-lacZ expression (blue) and desmin immunolocalization (brown). Desmin is an intermediate filament protein expressed early during myogenesis. Desmin staining is observed in the heart and is coexpressed in a small subpopulation of the Foxk1 promoter-lacZ-positive mesenchymal percursor cells of the limb. h, heart.