Figure 3.

Mutational analysis reveals a single β domain residue that is essential for EspP passenger domain cleavage. AD202 was transformed with a plasmid encoding EspPΔ1, EspP^*Δ1 or the indicated EspPΔ1 mutant, and autotransporter synthesis was induced by the addition of IPTG. Passenger domain cleavage was then analyzed by Western blot using the C-terminal antipeptide antiserum. (A) Analysis of serine, histidine and aspartate mutations. (B) Analysis of Asp¹¹²⁰ mutations. (C) Analysis of glutamate mutations.