Figure 8.

Role of lysines of G-CSFR in SOCS3-mediated inhibition of STAT5 activation. HEK293 cells were transfected with the indicated G-CSFR constructs, increasing amounts of SOCS3 (A, C), SOCS3 and SOCS3dBox (B), SOCS 2 (D) and SOCS1 (E) and a STAT5-responsive luciferase construct. SOCS protein levels were determined by Western blotting, of which a representative analysis is shown in Supplementary Figure 6. For each G-CSFR construct, G-CSF-induced luciferase reporter activity in the absence of SOCS was set at 100%. Data are representative of three independent experiments each performed in triplicate.