Figure 8.

Ring constriction during cytokinesis is delayed in hob3Δ cells. Analysis of contractile ring closure at 25°C. Time-lapse fluorescence microscopy of GFP-cdc4 in (A) hob3Δ and (B) wild type cells stained with calcofluor. (C) Time-lapse images of Hht2-GFP and GFP-cdc15 in wild-type and hob3Δ cells examined by confocal microscopy. (D) Rate of ring constriction at 25°C of wild-type (n=10) and hob3Δ cells (n=10) calculated from the time-lapse confocal images in three independent experiments.