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Seh1 depletion induces MT-dependent stripping of CENP-F. Cells transfected with control or Seh1 siRNAs were either fixed and stained with anti-Hec1 and anti-CENP-F antibodies and DAPI (A), or treated for 3 h with 20 μM nocodazole before fixation (B). Three-fold enlargements of the marked areas are presented. Scale bar, 5 μm.
