ChIP assays of wild type and skb1-1 mutant at the FLC locus. (A) A diagram of the FLC gene structure, with bars representing the A–E regions examined by ChIP. (B, C) ChIP results with antibodies against SKB1, H4R3sme2, H3K4me2, H3K9me2, H4R3me2 and acetylated H3K14 (H3K14ac). Samples were from wild-type and skb1-1 plants in LD for 20 days. The input is chromatin before immunoprecipitation. ‘No AB': the control sample lacks an antibody. ChIP assays involved at least three independent experiments; data represent results of one experiment.