Figure 4.

CLSM reveals distinct intracellular pools of 5-LO protein in naïve LCs. Epidermis was fixed and prepared as described under Methods. (A–D) Colocalization of lamin (Cy3) and 5-LO, LO32, antisera (Cy2). (E–H) Colocalization of DNA binding dye propidium iodide (red) and 5-LO, LO 32 Cy2. (A–D) Eight planes with distances of 0.9 μm each were recorded; planes 2, 4, 6, and 8 are shown. (E–H) Eleven planes with distances of 1 μm each were recorded; planes 2, 5, 8, and 11 are shown. (Bars = 2 μm.)