Table 1.
RT-PCR analyses of LCs, ECs − LCs, and human leukocyte buffy coat
| PCR product | LCs | ECs − LCs | Buffy coat |
|---|---|---|---|
| 5-LO | +++ | − | +++ |
| FLAP | +++ | + | +++ |
| LTA4 hydrolase | +++ | +++ | +++ |
| LTC4 synthase | ++ | − | + |
| CD1a | +++ | + | ++ |
| Tryptase | − | + | ++ |
| CD16 | − | − | +++ |
| Keratin 10 | + | +++ | − |
For preparation of ECs and RNA, and RT-PCR, see Materials and Methods. +++, Detectable at 28 cycles; ++, detectable at 34 cycles; +, detectable at 40 cycles; −, not detectable at 40 cycles. Markers: CD1a, LCs; tryptase, mast cells; CD16, natural killer cells, neutrophils, and macrophages; keratin 10, keratinocytes.