Figure 6. Western Blots and TLC Scanning Results for Rab28 with Radioactive Prenyl Anchor Precursors.

Western blot and corresponding scans from TLC linear analyzer of wild-type GST-Rab28-fusion protein translated with [³H]mevalonic acid (lane 1), GST-Rab28 C218A with [³H]mevalonic acid (lane 2), GST-Rab28 with [³H]FPP (lane 3), and GST-Rab28 with [³H]GGPP (lane 4). There is significant incorporation of a product of mevalonic acid as well as FPP, while incorporation of GGPP is not detectable, suggesting that Rab28 is primarily a farnesylation target.