Figure 7. Western Blots and TLC Scanning Results for FLJ32421 (BROFTI) with Radioactive Prenyl Anchor Precursors.

Western blot and corresponding scans from TLC linear analyzer of wild-type GST-FLJ32421-fusion protein translated with [³H]mevalonic acid (lane 1), GST-FLJ32421 C408A with [³H]mevalonic acid (lane 2), GST-FLJ32421 with [³H]FPP (lane 3) and GST-FLJ32421 with [³H]GGPP (lane 4). There is significant incorporation of a product of mevalonic acid as well as FPP, while incorporation of GGPP is close to the detection limit, suggesting that FLJ32421 (BROFTI) is primarily a farnesylation target.