Figure 9. Western Blots and TLC Scanning Results for a 15 Amino Acid C-terminal Fragment of Prickle2 with Radioactive Prenyl Anchor Precursors.

Western blot and corresponding scans from TLC linear analyzer of wild-type GST-ΔPrickle2-fusion protein translated with [³H]mevalonic acid (lane 1), GST-ΔPrickle2 C842A with [³H]mevalonic acid (lane 2), GST-ΔPrickle2 with [³H]FPP (lane 3), and GST-ΔPrickle2 with [³H]GGPP (lane 4). There is significant incorporation of a product of mevalonic acid as well as FPP, while incorporation of GGPP is lower despite a higher total amount of protein, suggesting that Prickle2 is primarily a farnesylation target.