Figure 2.

Reduction of PRC2 activity causes progressive defects in dendrite arborization. (A–E) Live imaging of class IV ddaC neurons visualized with the pickpocket-EGFP reporter. Images show wild-type (wt) (A) or esc²¹ homozygous mutant (B) larvae at 48 h AEL, and wild-type (C) or esc²¹ homozygous mutant (D–F) larvae at 96 h AEL. The arrow in E points to a major dendritic trunk that has been severed close to the cell body. Dorsal is up, and anterior is left. Bar, 50 μm. (F,G) Quantification of ddaC dendrite branch number (F) and total dendrite length (G) at 48 and 96 h AEL in wild-type and esc²¹ mutant larvae. Error bars represent one standard deviation, and asterisks (*) denote p < 0.05 relative to wild-type controls.