Figure 7.
Hox genes regulate terminal dendrite dynamics. Time-lapse imaging of representative control (A–C) or ScrC1, AntpNs + RC3, UbxMX12−/− (D–F) class IV ddaC MARCM clones. Clones were imaged at 80 h AEL (A,D) and again at 100 h AEL (B,E), and branch dynamics are depicted in traces (C,F). The dynamic behavior of terminal dendrites was assessed for a 150 × 200-μm region in the 100-h time point with the soma centered on the AP axis and the region of interest extending 200 mm dorsally. Comparable portions of this region are shown for representative clones in A–F. (C,F) Dynamics are represented in traces of 100-h dendrites. Terminal dendrites were scored as stable (colored black) if they were the same length in both images and were scored as dynamic if the terminal branch length increased via growth/branch initiation (green) or decreased via branch retraction/elimination (red). Terminal dendrite dynamics were measured from time-lapse images of nine control or mutant MARCM clones, and the mean total number of dendrite terminals (G), ratio of stable dendrite terminals (H), dynamic dendrite terminals (I), growing dendrite terminals (J), and retracting dendrite terminals (K) for MARCM clones of each genotype are shown. Error bars represent one standard deviation, and asterisks (*) denote p < 0.05 relative to wild-type controls.