Figure 8.

PcG genes interact with wts to regulate dendrite development. (A–C) Live image of third instar class IV ddaC neuron visualized using pickpocket-Gal4, UAS-mCD8∷GFP in wild-type (A), wts^latsX1/+ heterozygous, (B) or wts^latsX1/Pc³ trans-heterozygous (C) larva. Anterior is left, and dorsal is up. Bar, 50 μm. (D) Quantitation of dendrite branchpoints normalized to total dendritic length in ddaC of wild-type, wts^latsX1/+, sav³/+, hpo^mgh4/+, wts^latsX1/Pc³, sav³/Pc³, or hpo^mgh4/Pc³, wts^latsX1, Pc³/Scr^C1, Antp^{Ns + RC3}, or Ubx^MX12 or Scr^C1, Antp^{Ns + RC3}, Ubx^MX12/+ larvae. Error bars represent one standard deviation, and asterisks (*) denote p < 0.05 relative to wild-type controls. (E) Quantitation of the mean pixel intensity of anti-Ubx staining in ddaC neurons. wts^latsX1/+ heterozygous ddaC neurons that were located contralateral to wts^latsX1/wts^latsX1 homozygous ddaC clones were used for controls; n = 5. (F) Complex formation by PcG proteins and Wts. Drosophila S2 cells were transfected with Wts-HA alone (lane 1), Wts-HA and ESC-Flag (lane 2), or Wts-HA and Pc-Flag (lane 3), and the lysates were immunoprecipitated with anti-HA antibody. Representative Western blots of the immunoprecipitates (left panel; probed with anti-Flag antibody) and lysates used as input for the immunoprecipitates (right panel; probed with anti-Flag or anti-HA antibody) are shown.