Skip to main content
. Author manuscript; available in PMC: 2008 Feb 2.
Published in final edited form as: J Mol Biol. 2006 Oct 17;365(5):1407–1416. doi: 10.1016/j.jmb.2006.10.041

Figure 2.

Figure 2

Schematic representation of the experiment with protein immobilization approach for studying presynaptic SfiI-DNA complex. (a) AFM tip was modified with oligoduplexes and SfiI was immobilized on the surface. (b) Approach the tip to the surface to form the complex. (c) Application of pulling force results in the rupture of the presynaptic complex. (d) High concentration of target molecules results in the rupture of multiple contacts. (e, f) Addition of free DNA duplex to the solution increases probability of single complex rupture due to blocking active SfiI sites.