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. Author manuscript; available in PMC: 2007 Apr 5.
Published in final edited form as: Nat Struct Mol Biol. 2006 Apr 9;13(5):436–443. doi: 10.1038/nsmb1082

Figure 1.

Figure 1

Coulomb force dominates the interaction between restrictocin and oligonucleotides. (a) Structure of the rat SRL oligonucleotide substrate (PDB entry 430D). (b) KCl concentration dependence of SRL and pGpA cleavage under kcat/Km and kcat conditions. For kcat/Km conditions, reactions contained 0.001-10 μM restrictocin and <1 nM SRL. For kcat conditions, reactions contained 100 nM restrictocin and 5 μM SRL. The SRL data were fit to equation (1) (Methods). (c) Dependence of restrictocin binding (K½) on oligonucleotide length (Supplementary Table 2). K½ values were obtained from reactions containing 0.001-10 μM restrictocin, <1 nM RNA substrates and 5 mM KCl.