Skip to main content
. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2006 Nov 18;1768(2):324–335. doi: 10.1016/j.bbamem.2006.11.009

Figure 3.

Figure 3

Substitution of H1486 with either a leucine (L) or a phenylalanine (F) decreased the rate of ATP hydrolysis at the mutated NBD2. Membrane vesicles were prepared from Sf21 cells infected with viral particles containing either wild-type or variant mutants of human MRP1. Photolabeling experiments were carried out in a 10 μ l of solution containing 10 μ g membrane vesicle proteins, 800 μ M vanadate and 10 μ M of either [α -32P]-8-N3ATP (α ) or [γ -32P]-8-N3ATP (γ ) at 37 °C for 2 minutes. The reaction mixture was brought back to ice, UV-irradiated on ice for 2 minutes, subjected to SDS-PAGE (7%) and electroblotted to a nitrocellulose membrane. Molecular weight markers are indicated on the left. NH and CH on the right of the gel indicate the 32P-8-N3ATP labeled N-half and C-half of MRP1 proteins.