Effect of calcium manipulation on cytotoxicty of AA in CYP2E1-overexpressing liver cells. E47 cells (A), or primary rat liver cells from pyrazole-treated rats (B) were plated onto 24-well plates, and preincubated for 1h with MEM, SMEM, or SMEM with 5 μ M thapsigargin + 100 nM ionomycin. After this, cells were washed with PBS, and culture medium was added back (MEM if preincubated with MEM, or SMEM if preincubated in SMEM). AA (0 or 20 μ M) was added to the medium, and after 12h, viability was evaluated by the MTT test. *Significantly different (p<0.05) with respect to cells incubated in the same conditions, without AA.