FIG. 3.
Corrected dose–response curve for free radical (FR) trapping by pUC18 plasmid film hydrated to a Γ = 2.5 mol water/mol nucleotide fitted to the two-component model described previously (30). X irradiation and EPR were done at 4 K. Note that the slope shown in the inset (G) is the yield obtained from a fit to the initial linear response measured at low dose (~6 kGy). The free radical yield, G(∑fr), of this pUC18 plasmid (2686 bp) sample determined from the fit is found to be 0.285 ± 0.006 μmol/J. R2 (~0.998) is the goodness of fit and χ2 (~7.04 × 1028) is the reduced χ2 value calculated by the nonlinear least-squares fitting routine used by ORIGIN.