Table 1.

Enrichment of anti-mesothelin phage over three rounds of panning at 37°C by using direct or off-rate selection

Panning round (selection)	Input no. of phage	No. of phage binding	No. of mesothelin positive phage/ no. tested
1 Direct	2  × 1011	7  × 104	0/48
Off-rate	2  × 1011	2  × 104	0/48
2 Direct	7  × 1010	8  × 104	2/48
Off-rate	3  × 1010	8  × 103	1/48
3 Direct	1  × 1010	6  × 106	32/48
Off-rate	3  × 109	4  × 105	30/48